The Royal Institution for the Advancement of Learning/McGill University

Published purpose

Adeno-associated virus production is typically carried out by transient transfection of 293 cells. It is therefore important to identify the molecular pathways in these cells that are critical for high-level viral production. This project will thus undertake a genome-wide screen for such host genes. The experimental strategy relies upon the up-regulation of cellular genes by CRISPR-dCasa and identification of those genes, whose overexpression results in an increase in AAV production. The long-term goal of this project would be to use this valuable information to genetically engineer 293SF-3F6 cells with the aim of generating a producer cell that has the ability to support high-level AAV production