Centre hospitalier universitaire Sainte-Justine

Published purpose

Since the beginning of the COVID pandemic, the analysis of viral sequences isolated from patient samples has become essential for tracking virus variants and determining which mutations affect the viral proteins and influence its pathogenicity or its infectiousness. Purification of the viral particles prior to the required RNA sequencing would reduce the cost of sequencing and is less biased than alternative methods based on targeted sequencing or amplification of the viral genome. In this Project, the Recipient aims to develop protocols to optimize the purification of viral particles on the PowerBlade Platform, and subsequently, through sequencing, develop protocols for the identification of viral strains on the device.