Novel Biotechnology Inc.

Published purpose

Novel has developed a novel platform for producing plasmids (NBX). Cloning is the process used to create a bacterial vector containing a gene of interest. There are several industry-standard procedures available for cloning, including restriction digestion-ligation-based cloning, Gibson assembly, and Golden Gate assembly. Once complete, the cloned plasmid is introduced into a bacterial cell (NBX) using a procedure called transformation. It is important to determine an optimized recipe for the transformation mixture based on the cloning mixture and heat shock parameters based on the format of the reaction tube. The trainee will work on these aspects, gaining experience in cloning/assembly methods transformation methods and recovery methods.